Journal: Physiological Reports

Article Title: Dietary fats modify vascular fat composition, eNOS localization within lipid rafts and vascular function in obesity

doi: 10.14814/phy2.13820

Figure Lengend Snippet: (A) Representative western immunoblots of eNOS , iNOS , Cox1, Cox2, and actin in whole cell lysate from gracilis arteries from WT mice on LF , HF , and MO ( n = 3 each). (B) Expression of eNOS , iNOS , Cox1, and Cox2 normalized to actin (Mean ± SEM , n = 6 each). There were no significant differences in expression of any protein with diet. One‐way ANOVA.

Article Snippet: After blocking, immunoblotting was performed with antibodies to eNOS (1:500, BD Transduction, #160297), p‐eNOS Ser1177 (1:250, Cell Signaling, #9571), iNOS, (1:500, Boster, #PA1330‐1), Caveolin‐1 (1:500, BD Sciences, #610407), cyclooxygenase‐1 (1:500, Boster, #M00811), cyclooxygenase‐2 (1:500, Boster, #M00084), and β ‐actin (1:600, Sigma‐Aldrich, #A2228) followed by secondary antibodies conjugated to horseradish peroxidase and analyzed using Image J (NIH).

Techniques: Western Blot, Expressing

Journal: Physiological Reports

Article Title: Dietary fats modify vascular fat composition, eNOS localization within lipid rafts and vascular function in obesity

doi: 10.14814/phy2.13820

Figure Lengend Snippet: (A) Representative western immunoblots of eNOS , Ser1177 p‐ eNOS , iNOS , Cox1, Cox2, Cav‐1, and actin in lipid raft fractions from WT LF aorta under basal conditions and following relaxation to AC h (1 μ mol/L). Only eNOS was localized to low‐density cav‐1 containing fractions under basal conditions which shifted to high‐density fractions in AC h‐stimulated aorta. Expression of eNOS (B), iNOS (C), Cox1 (D), Cox 2 (E), Cav‐1 (F), and actin (G) as a percent of total in low‐density fractions (1) to heavy density fractions (9) of aorta from WT mice on LF diet under basal conditions or following relaxation to acetylcholine ( AC h, 1 μ mol/L). eNOS was primarily localized to cav‐1 containing low‐density fractions under basal conditions which shifted to heavy fractions with AC h. iNOS , Cox1, and Cox2 were not localized to low‐density cav‐1 containing fractions or affected by AC h. The percent of actin in heavy density fractions increased with AC h (G). Mean ± SEM . Two‐tailed unpaired t test.

Article Snippet: After blocking, immunoblotting was performed with antibodies to eNOS (1:500, BD Transduction, #160297), p‐eNOS Ser1177 (1:250, Cell Signaling, #9571), iNOS, (1:500, Boster, #PA1330‐1), Caveolin‐1 (1:500, BD Sciences, #610407), cyclooxygenase‐1 (1:500, Boster, #M00811), cyclooxygenase‐2 (1:500, Boster, #M00084), and β ‐actin (1:600, Sigma‐Aldrich, #A2228) followed by secondary antibodies conjugated to horseradish peroxidase and analyzed using Image J (NIH).

Techniques: Western Blot, Expressing, Two Tailed Test

Journal: Scientific Reports

Article Title: A comprehensive phenotypic and genotypic evaluation of Spanish groundnuts from diverse crosses to identify superior and stable donors for fresh seed dormancy

doi: 10.1038/s41598-024-64681-6

Figure Lengend Snippet: Comparative ranks of AMMI stability index (ASI) and simultaneous selection index (SSI) for yield and related traits in groundnut.

Article Snippet: Among other genotypes with predicted means above grand means from BLUP estimates were, PBS 16053, 16025, 16027, 16031, TPG 41, 16004, 15044, 16052, 16013, 16020, 16026, and 16029 for PYLP; PBS 15044, 16038, 16046, 16020, 16035, 16047, 15004, 16025, 16053, 16015, 16037, and 16024 for HPW; PBS 16016, 15044, 16053, 16037, 16021, 16015, Girnar 3, 16020, 16025, 16004, 16046 for HKW; PBS 16013, 16031, 16028, 16029, 16027, 16020, 16039, 16041, 16015, 15044, 16044, 16047, and 16045 for SP.

Techniques: Selection

Journal: Scientific Reports

Article Title: A comprehensive phenotypic and genotypic evaluation of Spanish groundnuts from diverse crosses to identify superior and stable donors for fresh seed dormancy

doi: 10.1038/s41598-024-64681-6

Figure Lengend Snippet: Estimates of YREM’s of groundnut genotypes for yield and related traits.

Article Snippet: Among other genotypes with predicted means above grand means from BLUP estimates were, PBS 16053, 16025, 16027, 16031, TPG 41, 16004, 15044, 16052, 16013, 16020, 16026, and 16029 for PYLP; PBS 15044, 16038, 16046, 16020, 16035, 16047, 15004, 16025, 16053, 16015, 16037, and 16024 for HPW; PBS 16016, 15044, 16053, 16037, 16021, 16015, Girnar 3, 16020, 16025, 16004, 16046 for HKW; PBS 16013, 16031, 16028, 16029, 16027, 16020, 16039, 16041, 16015, 15044, 16044, 16047, and 16045 for SP.

Techniques: